Home > Products > Surfactant
CasNo： 9002-93-1
MF： (C2H4O)nC14H22O
Appearance： Clear oily liquid
|
Description |
Triton X-100, chemically known as polyethylene glycol p-(1,1,3,3-tetramethylbutyl)phenyl ether, is a nonionic surfactant. It consists of a hydrophilic polyethylene oxide chain and a lipophilic aromatic hydrocarbon group, specifically a 4-phenyl group. Its structure is a polyethylene glycol derivative with one of the terminal hydroxy groups converted into the corresponding p-(2,4,4-trimethylpentan-3-yl)phenyl ether. |
|
Uses |
Triton X-100 is widely utilized as a detergent in laboratories due to its ability to solubilize proteins, lyse cells for extraction of protein or organelles, and permeabilize cell membranes. Triton X-100 is commonly used in biochemical applications for its non-denaturing properties on proteins while denaturing cell membranes. However, its use as a surfactant raises environmental concerns because it is non-biodegradable and can inhibit certain microbial organisms. Biodegradation of Triton X-100 has been observed in aerobic and anaerobic municipal wastewater sludge and specific microbial organisms. Adsorption and biodegradation are the mechanisms responsible for Triton removal from the environment. In addition, Triton X-100's insolubility in lipids is exploited for probing the structure of biological membranes. It is particularly useful for studying lipid bilayers with tight lipid packing. The Triton X-100-insoluble lipid fraction obtained from detergent extraction of eukaryotic cells consists of membranes rich in sphingolipids and cholesterol, which are often associated with lipid rafts. The degree of lipid insolubility depends on the stability of lipid-lipid interactions relative to lipid-detergent interactions and can vary based on factors such as lipid composition, detergent type, and temperature. |
|
Definition |
ChEBI: A poly(ethylene glycol) terminated with a 4-(2,4,4-trimethylpentan-3-yl) phenyl group at one end. |
InChI:InChI=1/C32H58O10/c1-2-3-4-5-6-7-10-31-11-8-9-12-32(31)42-30-29-41-28-27-40-26-25-39-24-23-38-22-21-37-20-19-36-18-17-35-16-15-34-14-13-33/h8-9,11-12,33H,2-7,10,13-30H2,1H3
We studied the mutual diffusion coefficient of Triton X-100: water solutions for a series of Triton X-100 concentrations between 5 and 100 g/L and temperatures in the range 10-50 C.
A simple procedure is described for removal of Triton X-100 from protein samples by adsorption of the detergent on a commercial copolymer in bead form. The concentration of detergent can be lowered to approximately 0.01% with no loss or dilution of protein.
Yeast cells are completely permeabilized by Triton X-100 concentrations of 0.05% (v/v), and permeabilization is independent of cell age and cell concentration. The treatment makes the cells freely diffusible for macromolecules up to molecular weights around 70,000.
